Poster électronique

Détection de l'activité macrophagique par IRM dans un modèle expérimental d'ostéomyélite vertébrale
G. Bierry, F. Jehl, N. Boehm, G. Prévost, M. Mohr, JL. Dietemann, S. Kremer - Strasbourg - France
Congrès 2007
Poster
0
0 votes.
Vu 9 fois.
Résumé
Objectifs
Evaluation des USPIO (Sinerem®) comme marqueur de l'activité macrophagique dans un modèle expérimental d'infection vertébrale.
Matériels et méthodes
Développement d'une ostéomyélite vertébrale chez 10 lapins par injection intra-discale de staphylocoques. Réalisation de deux sessions d'IRM (1,5 T), en pondération T1, T2 et T2* avant et 24 heures après injection de Sinerem® (45 mg Fe/kg). Analyse du ratio signal/bruit (RSB) des vertèbres infectées et saines avant et après injection. Corrélation histo-pathologique (marquage macrophagique, coloration de Perls).
Résultats
Vertèbres infectées : augmentation significative du RSB en séquence T1 (p = 0,005) sans modification significative du T2 et T2* (p = 0,14 et p = 0,87). Vertèbres saines : diminution significative du RSB en T1, T2 et T2*. Etude histo-pathologique : disparition de la moelle hématopoïétique des vertèbres infectées, remplacée par une importante infiltration macrophagique dont une faible proportion est marquée à la coloration Perls. Vertèbres saines : moelle hématopoïétique normale, sans infiltration macrophagique, avec important marquage au Perls.
Conclusion
L'IRM permet de montrer la présence de macrophages chargés en USPIO au niveau des zones infectées dans un modèle animal d'ostéomyélite vertébrale. La faible proportion de macrophages chargés en USPIO présents dans les zones infectées induit une augmentation significative du RSB en T1 absent dans les zones saines.
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2017-04-11 19.24 :
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